Registration of 252 sequenced sorghum mutants as a community reverse genetic resource

Induced mutagenesis is a powerful approach to generate variations that can be used toward the elucidation of gene function and create new traits for crop improvement. We have developed pedigreed mutant library through chemical with ethyl methanesulfonate (EMS)-treated seed from sorghum (Sorghum bicolor L. Moench) inbred line BTx623. The displayed wide diversity phenotypes, including potential significant agronomic value. A selection 252 lines (Reg. no. GS-794–Reg GS-1045, PI 701562–PI 701813) were sequenced an average depth 16x paired-end sequencing using Illumina Highseq. This endeavor resulted in detection cataloguing more than 1.8 million canonical EMS-induced mutations (variants). variants distributed across genome uniformly, 24,757 genes affected by impactful may alter function. Mutations interest searched online SorghumBase (https://www.sorghumbase.org). These mutants ordered GRIN (https://www.ars-grin.gov/) no associated fee.